Dna Extraction Lab Report Discussion
Dna extraction the concentrations of the extracted dna was measured using the nanophotometer uv vis spectrophotometer implen munich germany and the average concentration for 12 samples was 0 045µg µl.
Dna extraction lab report discussion. Activity 1 dna extraction. The following discussion points should be addressed in the appropriate section of the lab report. Describe what happens at each step and why it acts to separate the parts of the cell. Justify the use of salt soap and alcohol in the extraction procedure.
Dna extraction isolates dna strands from the cells of living organisms. Dna extraction of a musa acuminata biology lab report analyze and interpret a number of steps are required to isolate dna from cellular contents. The steps used to remove and expose dna from its cell are. Friedrich miescher discovered dna extraction in 1869 tamari and hinkley 2016 p240 and since that time researchers across the ages have employed the techniques to isolate dna materials from plants and animals for research.
We will extract dna from fruit to investigate how it looks and feels. Given the momentousness of the dna it is one area that has adequately intrigued many researchers. Conclusions and analysis of dna extraction lab answers sciencenorth ca schools science north is an agency of the government of ontario and a registered charity 10796 2979 rr0001. Results discussion 4 1.
This information can be used to improve crops so that they are more resistant to disease insect invasion or changes in climate. Extracting dna is important to scientists because this greatly aids scientists in finding cure to genetic disorders new cures to these illnesses in recent years are largely thanks to dna extraction cures such as bone marrow transplant for sickle cell disease. Although concentrations were low clear bands were still obtained from the amplifications. I used the alcohol extraction method to isolate dna and to make it visible to the naked eye.
Microsoft word extracting dna from peas conclusions and analysis of dna extraction lab answers docx. It can be concluded that from my experiment and the research that dna from different foods does vary and in appearance as well. I chose strawberries because they are easy to manipulate and they contain a large genome. With a pure sample of dna we can test a newborn for a genetic disease analyze forensic evidence or study a gene involved in cancer.
Discuss the structure of a plant cell. Strawberry dna extraction lab report. Gram negative bacteria are a group of bacteria that do not retain the crystal violet stain when rinsed with alcohol used in the gram staining method of bacterial differentiation 1 making positive. Dna is extracted from human cells for a variety of reasons.
6 there are three specific steps required to isolate dna from its cellular contents. The dna was extracted due to the dishwashing liquid detergent busts open the cells of the fruit releasing the dna and the salt helps as it ensures that the proteins in the cells are not separated from the rest of the solution with the dna. This procedure is similar to what scientists have to do before they can use the information contained in this dna.